PP88 No Further a Mystery
PP88 No Further a Mystery
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23. A nucleic acid vector for use in in vivo shipping of a nucleic acid of interest into a targeted receiver bacterial cell, said nucleic acid of curiosity manufacturing a offered effect on claimed targeted receiver bacterial mobile, wherein reported vector comprises: explained nucleic acid of curiosity, and
whereby, once sent into explained focused receiver bacterial cell, claimed nucleic acid of curiosity provides claimed specified impact on explained qualified receiver bacterial mobile when reported vector is not replicated in explained qualified receiver bacterial mobile.
a conditional origin of replication which happens to be inactive from the targeted receiver bacterial cell but is Energetic in a donor bacterial cell, and
Restriction enzymes are generally categorized into four types (forms I to type IV). The REBASE database enable to checklist the restriction web-sites that a specified bacterium can acknowledge based on the restriction enzymes that it expresses.
comprising stated nucleic acid of interest, wherein reported vector even further comprises a conditional origin of replication that's inactive inside the focused receiver bacterial cell but is Lively in the donor bacterial mobile, and reported vector is devoid of antibiotic resistance marker,
In a certain embodiment, the subject has never obtained any remedy before the administration of your vectors based on the creation, notably a vector packaged into a shipping car or truck according to the creation, preferably a packaged plasmid or phagemid right into a bacterial virus particle based on the invention, or of a pharmaceutical or veterinary composition according to the invention.
especially, the packaging website of claimed vector just isn't derived with the organism from which the conditional origin of replication is derived.
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In a certain embodiment, reported given result is making the receiver bacterial cell quit generating a presented molecule.
mentioned molecule of interest may well even more be produced by claimed focused receiver bacterial cell in any sort. specifically, mentioned HMM may very well be selected in the group consisting of secreted molecules, intracellular molecules and membrane-displayed molecules.
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These gene sequences may be modified to lessen the homology Using the human sequences and stop cross-reactivity of Those people regarded through the host immune technique Along with the human counterpart.
As applied herein, the terms “restriction enzyme” and “restriction endonuclease” are equal and make reference to an enzyme that cuts nucleic acids at or in close proximity to restriction web-sites.
The inventors display for The 1st time that phagemids can be packaged at large titers with a conditional ORI with ori and protein demanded for replication in trans,
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